Next generation LC-MS based proteomics - A new era of speed, scale and robustness

Liquid chromatography-mass spectrometry (LC-MS) is a cornerstone of modern proteomics research, revealing the intricate interactions of proteins driving cellular function and disease. For decades, exploratory efforts pushed the boundaries of protein identification and quantification, with landmark studies mapping the human proteome, and generating deep protein profiles of single cell lines1,2. However, these achievements required extensive measurement times, laborious sample fractionation, and substantial starting material. Gaining proteomic depth meant sacrificing throughput, and labs developed.

Specialized setups, which limited adoption by other research groups, hindered comparability, and precluded routine large-scale studies. Today, proteomics is undergoing a profound transformation. New technologies are overcoming these limitations, merging high proteome depth and throughput into standardized workflows. Among those advances is the Evosep Eno. Three early adopters share how this instrument prepares them for next-generation proteomics – the paradigm shift that will launch scalable and robust sample interrogation in application areas like systems biology and precision medicine.