Multi-Omics with 4D-TIMS Innovation.
Comprehensive investigation of biochemical and medical systems necessitates robust and scalable analysis of diverse molecular classes across a broad dynamic range of sample amounts. The timsMetabo™ mass spectrometer was designed to deliver unprecedented TIMS separation power for small molecules, while maintaining the established, high performance for bottom-up proteome sample analysis. We demonstrate that the timsMetabo is capable of addressing complex cell lysate samples from hundreds of nanograms to single cell levels, as well as comprehensively covering the plasma proteome for neat plasma preparations and enriched plasma protein fractions (ENRICH-iST). Furthermore, we present a new TIMS-enabled MS/MS mode which unlocks a larger dynamic loading range for complex proteomics samples